Tag-based next generation sequencing / (Record no. 205523)
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| 000 -LEADER | |
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| fixed length control field | 05609cam a2200601Mi 4500 |
| 001 - CONTROL NUMBER | |
| control field | ocn773564828 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OCoLC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20171115112138.0 |
| 006 - FIXED-LENGTH DATA ELEMENTS--ADDITIONAL MATERIAL CHARACTERISTICS | |
| fixed length control field | m o d |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION | |
| fixed length control field | cr |n|---||||| |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 120123s2011 nju ob 000 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9783527644582 |
| Qualifying information | (electronic bk.) |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 352764458X |
| Qualifying information | (electronic bk.) |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9783527644773 |
| Qualifying information | (electronic bk.) |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 3527644776 |
| Qualifying information | (electronic bk.) |
| 029 1# - OTHER SYSTEM CONTROL NUMBER (OCLC) | |
| OCLC library identifier | AU@ |
| System control number | 000048852576 |
| 029 1# - OTHER SYSTEM CONTROL NUMBER (OCLC) | |
| OCLC library identifier | DEBSZ |
| System control number | 431074321 |
| 029 1# - OTHER SYSTEM CONTROL NUMBER (OCLC) | |
| OCLC library identifier | NZ1 |
| System control number | 15922069 |
| 029 1# - OTHER SYSTEM CONTROL NUMBER (OCLC) | |
| OCLC library identifier | DEBBG |
| System control number | BV043394030 |
| 035 ## - SYSTEM CONTROL NUMBER | |
| System control number | (OCoLC)773564828 |
| Canceled/invalid control number | (OCoLC)784138844 |
| 037 ## - SOURCE OF ACQUISITION | |
| Stock number | 10.1002/9783527644582 |
| Source of stock number/acquisition | Wiley InterScience |
| Note | http://www3.interscience.wiley.com |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EBLCP |
| Language of cataloging | eng |
| Description conventions | pn |
| Transcribing agency | EBLCP |
| Modifying agency | OCLCQ |
| -- | UIU |
| -- | N$T |
| -- | DG1 |
| -- | YDXCP |
| -- | OCLCQ |
| -- | OHI |
| -- | OCLCF |
| -- | UKDOC |
| -- | OCLCQ |
| -- | DEBSZ |
| -- | OCLCQ |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | MAIN |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER | |
| Classification number | QP624.5.D726 .H889 2011 |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | SCI |
| Subject category code subdivision | 029000 |
| Source | bisacsh |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 572.8633 |
| 245 00 - TITLE STATEMENT | |
| Title | Tag-based next generation sequencing / |
| Statement of responsibility, etc. | edited by Matthias Harbers and Günter Kahl. |
| Medium | [electronic resource] |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | Hoboken : |
| Name of publisher, distributor, etc. | John Wiley & Sons, |
| Date of publication, distribution, etc. | 2011. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 1 online resource (609 pages) |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Content type code | txt |
| Source | rdacontent |
| 337 ## - MEDIA TYPE | |
| Media type term | computer |
| Media type code | c |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | online resource |
| Carrier type code | cr |
| Source | rdacarrier |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Tag-based Next Generation Sequencing; Contents; Preface; List of Contributors; Part One: Tag-Based Nucleic Acid Analysis; 1 DeepSuperSAGE: High-Throughput Transcriptome Sequencing with Now- and Next-Generation Sequencing Technologies; 1.1 Introduction; 1.2 Overview of the Protocols; 1.2.1 Principle of the SuperSAGE Method; 1.2.2 Power of the SuperSAGE Tag; 1.2.3 Development of DeepSuperSAGE; 1.2.4 Ditag-Based DeepSuperSAGE (for 454 Pyrosequencing); 1.2.5 Single-Tag-Based DeepSuperSAGE (HT-SuperSAGE); 1.3 Methods and Protocols; 1.3.1 Linker or Adapter Preparation; 1.3.2 RNA Samples. |
| 505 8# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | 1.3.3 cDNA Synthesis and NlaIII Digestion; 1.3.4 Tag Extraction from cDNA; 1.3.5 Tag Extraction from cDNA; 1.3.6 Purification of Linker-Tag Fragments; 1.3.7 Ditag or Adapter-Tag Formation and Amplification; 1.3.8 Preparation of Templates for Sequencing; 1.4 Applications; 1.4.1 Applications of DeepSuperSAGE in Combination with 454 Pyrosequencing; 1.4.2 Practical Analysis of HT-SuperSAGE; 1.5 Perspectives; References; 2 DeepCAGE: Genome-Wide Mapping of Transcription Start Sites; 2.1 Introduction; 2.2 What is CAGE?; 2.3 Why CAGE?; 2.4 Methods and Protocols; 2.4.1 Key Reagents and Consumables. |
| 505 8# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | 2.4.2 Precautions; 2.4.3 RNA Samples Used for DeepCAGE Library Preparation; 2.4.4 DeepCAGE Library Preparation; 2.5 Applications; 2.6 Perspectives; References; 3 Definition of Promotome-Transcriptome Architecture Using CAGEscan; 3.1 Introduction; 3.2 What is CAGEscan?; 3.3 Why CAGEscan?; 3.4 Methods and Protocols; 3.4.1 Key Reagents and Consumables; 3.4.2 Precautions; 3.4.3 RNA Samples Used for CAGEscan Library Preparation; 3.4.4 Considerations on Pooling CAGEscan Libraries; 3.4.5 CAGEscan Library Preparation; 3.5 Applications and Perspectives; References. |
| 505 8# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | 4 RACE: New Applications of an Old Method to Connect Exons; 4.1 Introduction; 4.2 Deep-RACE; 4.2.1 Choice of the Sequencer; 4.2.2 Validation of Promoter Studies; 4.2.3 Other Applications of Deep-RACE; 4.2.4 Limitations of Deep-RACE; 4.3 Methods Outline; 4.3.1 Primer Design; 4.3.2 Molecular Biology of Deep-RACE Library Preparation; 4.3.3 Sequencing of Deep-RACE Libraries; 4.3.4 Analysis; 4.4 Perspectives; References; 5 RNA-PET: Full-Length Transcript Analysis Using 5'- and 3'-Paired-End Tag Next-Generation Sequencing; 5.1 Introduction; 5.2 Methods and Protocols. |
| 505 8# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | 5.2.1 Key Reagents and Consumables; 5.2.2 Protocol; 5.3 Applications; 5.3.1 PET Sequencing with SOLiD; 5.3.2 Mapping of the PETs; 5.3.3 PET Clustering, Annotation, and Genome Browser Visualization; 5.4 Perspectives; References; 6 Stranded RNA-Seq: Strand-Specific Shotgun Sequencing of RNA; 6.1 Introduction; 6.1.1 Before Starting; 6.2 Methods and Protocols; 6.2.1 Preface; 6.2.2 Materials and Consumables; 6.2.3 Protocol; 6.3 Bioinformatic Considerations; 6.4 Applications; 6.5 Perspectives; References. |
| 505 8# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | 7 Differential RNA Sequencing (dRNA-Seq): Deep-Sequencing-Based Analysis of Primary Transcriptomes. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Tag-based approaches were originally designed to increase the throughput of capillary sequencing, where concatemers of short sequences were first used in expression profiling. New Next Generation Sequencing methods largely extended the use of tag-based approaches as the tag lengths perfectly match with the short read length of highly parallel sequencing reactions. Tag-based approaches will maintain their important role in life and biomedical science, because longer read lengths are often not required to obtain meaningful data for many applications. Whereas genome re-sequencing and de novo sequ. |
| 588 0# - SOURCE OF DESCRIPTION NOTE | |
| Source of description note | Print version record. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | DNA microarrays. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Neurobiology. |
| 650 #4 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Oligonucleotide array sequence analysis. |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | SCIENCE |
| General subdivision | Life Sciences |
| -- | Genetics & Genomics. |
| Source of heading or term | bisacsh |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | DNA microarrays. |
| Source of heading or term | fast |
| Authority record control number | (OCoLC)fst00886592 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Neurobiology. |
| Source of heading or term | fast |
| Authority record control number | (OCoLC)fst01036315 |
| 655 #4 - INDEX TERM--GENRE/FORM | |
| Genre/form data or focus term | Electronic books. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Harbers, Matthias. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Kahl, Günter. |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Print version: |
| Title | Tag-based Next Generation Sequencing. |
| Place, publisher, and date of publication | Hoboken : John Wiley & Sons, ©2011 |
| International Standard Book Number | 9783527328192 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | http://onlinelibrary.wiley.com/book/10.1002/9783527644582 |
| Public note | Wiley Online Library |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | |
| Koha item type | Books |
No items available.