PCR guru : an ultimate benchtop reference for molecular biologists / (Record no. 247486)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 05538cam a2200601Ii 4500 |
| 001 - CONTROL NUMBER | |
| control field | ocn965140915 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OCoLC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20190328114817.0 |
| 006 - FIXED-LENGTH DATA ELEMENTS--ADDITIONAL MATERIAL CHARACTERISTICS | |
| fixed length control field | m o d |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION | |
| fixed length control field | cr |n||||||||| |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 161202t20172017ne ob 001 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | YDX |
| Language of cataloging | eng |
| Description conventions | rda |
| -- | pn |
| Transcribing agency | YDX |
| Modifying agency | OCLCQ |
| -- | NLE |
| -- | OCLCO |
| -- | IDEBK |
| -- | OPELS |
| -- | N$T |
| -- | EBLCP |
| -- | GZM |
| -- | STF |
| -- | IDB |
| -- | DEBSZ |
| -- | OCLCF |
| -- | TFW |
| -- | N$T |
| -- | GZM |
| -- | OCLCQ |
| -- | VGM |
| -- | OCLCQ |
| -- | OCL |
| -- | MERUC |
| -- | CNCGM |
| -- | U3W |
| -- | UAB |
| -- | COO |
| -- | OTZ |
| -- | OCLCO |
| -- | OCL |
| -- | MERER |
| -- | ORE |
| -- | OCLCO |
| -- | D6H |
| -- | OCLCQ |
| -- | CHVBK |
| -- | BRX |
| -- | OCLCQ |
| -- | OCLCO |
| -- | WYU |
| -- | OCLCO |
| -- | OCLCA |
| 016 7# - NATIONAL BIBLIOGRAPHIC AGENCY CONTROL NUMBER | |
| Record control number | 101702242 |
| Source | DNLM |
| 019 ## - | |
| -- | 964656833 |
| -- | 967076811 |
| -- | 971588839 |
| -- | 971589167 |
| -- | 971918018 |
| -- | 1066596587 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9780128042465 |
| Qualifying information | (electronic bk.) |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 012804246X |
| Qualifying information | (electronic bk.) |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| Canceled/invalid ISBN | 0128042311 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| Canceled/invalid ISBN | 9780128042311 |
| 035 ## - SYSTEM CONTROL NUMBER | |
| System control number | (OCoLC)965140915 |
| Canceled/invalid control number | (OCoLC)964656833 |
| -- | (OCoLC)967076811 |
| -- | (OCoLC)971588839 |
| -- | (OCoLC)971589167 |
| -- | (OCoLC)971918018 |
| -- | (OCoLC)1066596587 |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER | |
| Classification number | QP606.D46 |
| 060 00 - NATIONAL LIBRARY OF MEDICINE CALL NUMBER | |
| Classification number | 2017 B-894 |
| 060 14 - NATIONAL LIBRARY OF MEDICINE CALL NUMBER | |
| Classification number | QU 25 |
| Item number | N162p 2017 |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | SCI |
| Subject category code subdivision | 007000 |
| Source | bisacsh |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 572.33 |
| Edition number | 23 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Najafov, Ayaz, |
| Relator term | author. |
| 245 10 - TITLE STATEMENT | |
| Title | PCR guru : an ultimate benchtop reference for molecular biologists / |
| Medium | [electronic resource] |
| Statement of responsibility, etc. | Ayaz Najafov, Gerta Hoxhaj. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Place of production, publication, distribution, manufacture | Amsterdam : |
| Name of producer, publisher, distributor, manufacturer | Academic Press, Elsevier, |
| Date of production, publication, distribution, manufacture, or copyright notice | 2017 |
| 264 #4 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Date of production, publication, distribution, manufacture, or copyright notice | �2017 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 1 online resource (xv, 89 pages) |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Content type code | txt |
| Source | rdacontent |
| 337 ## - MEDIA TYPE | |
| Media type term | computer |
| Media type code | c |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | online resource |
| Carrier type code | cr |
| Source | rdacarrier |
| 500 ## - GENERAL NOTE | |
| General note | Publisher, Sara Tenney ; acquisitions editor, Sara Tenney ; editorial project manager, Fenton Coulthurst ; production project manager, Chris Wortley ; designer, Christian Bilbow. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc | Includes bibliographical references pages 83-86) and index. |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Cover; Title Page; Copyright Page; Dedication; Contents; Biography; Preface; Acknowledgments; Note to the Reader; Chapter 1 -- Introduction; Abstract; 1.1 -- A bit of history; 1.2 -- What is PCR?; 1.3 -- PCR components; 1.4 -- Exponential nature of PCR; 1.5 -- Popularity of PCR; Chapter 2 -- Procedure; Abstract; 2.1 -- Primer design; 2.2 -- Standard PCR setup; 2.3 -- Controls; 2.4 -- Cycling parameters; 2.4.1 Denaturation; 2.4.2 Annealing; 2.4.3 Extension; Chapter 3 -- Good PCR Practices; Abstract; Chapter 4 -- Optimization and Troubleshooting; Abstract; 4.1 -- Optimization rules. |
| 505 8# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | 4.2 -- General optimization strategies4.2.1 -- Annealing Temperature Gradient; 4.2.2 -- Mg++ Titration; 4.2.3 -- Hot Start; 4.2.4 -- Touchdown PCR; 4.2.5 -- Additives; 4.3 -- Troubleshooting specific problems; 4.3.1 -- Problem Type 1; 4.3.2 -- Problem Type 2; 4.3.3 -- Problem Type 3; 4.3.4 -- Problem Type 4; 4.3.5 -- Problem Type 5; 4.3.6 -- Other Problem Types; Chapter 5 -- Tips and Tricks; Abstract; Chapter 6 -- Special Cases; Abstract; Glossary and Abbreviations; A; B; C; D; E; G; K; M; N; O; P; Q; R; S; T; X; Appendix A -- Agarose Gel Electrophoresis Protocol; Appendix B -- Silver Staining of DNA Gels. |
| 505 8# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Appendix C -- Plasmid Miniprep Protocol (by using "QIAprep Spin Miniprep" Kit)Appendix D -- Restriction Enzyme Digestion Protocol; Appendix E -- Protocol for DNA Concentration Estimation Using Hoechst 33258; Appendix F -- Formulas; Appendix G -- Common Primers for Sequencing, Promoters, Housekeeping Genes, and Reporters; Appendix H -- Tags; Appendix I -- Thermostable DNA polymerases; Appendix J -- Example of a Daily PCR Log; Appendix K -- Common Buffers and Solutions; Appendix L -- Useful Numbers; Appendix M -- Yeast Colony PCR; Useful Websites; References; Index; Back Cover. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | "PCR Guru: An Ultimate Benchtop Reference for Molecular Biologists is provides researchers in molecular biology with a handy reference for approaching and solving challenging problems associated with PCR setup and optimization. As a laboratory guide, it emphasizes the technical aspects of employing PCR as a tool in molecular biology laboratories. The book covers the history of PCR and the basic science underlying it. It then discusses PCR at the bench level, starting with detailed description and tips on primer design, and continuing with the standard protocols used to perform PCR. Provides troubleshooting tips for various types of modifications of standard protocols Contains unique "Good Practices and Tips that are indispensable for the beginner and expert alike Features "Special Cases with applications of PCR, optimization, and troubleshooting Includes detailed appendices with tables, figures, and key protocols Organized as a systematic, concentrated resource to save time when addressing a PCR problem." |
| 588 0# - SOURCE OF DESCRIPTION NOTE | |
| Source of description note | Online resource; title from PDF title page (ScienceDirect, viewed February 16, 2017). |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Polymerase chain reaction. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Molecular biology. |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | SCIENCE |
| General subdivision | Life Sciences |
| -- | Biochemistry. |
| Source of heading or term | bisacsh |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Molecular biology. |
| Source of heading or term | fast |
| Authority record control number | (OCoLC)fst01024734 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Polymerase chain reaction. |
| Source of heading or term | fast |
| Authority record control number | (OCoLC)fst01070553 |
| 650 12 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Polymerase Chain Reaction |
| General subdivision | methods. |
| Authority record control number | (DNLM)D016133Q000379 |
| 650 22 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Molecular Biology. |
| Authority record control number | (DNLM)D008967 |
| 655 #4 - INDEX TERM--GENRE/FORM | |
| Genre/form data or focus term | Electronic books. |
| 655 #0 - INDEX TERM--GENRE/FORM | |
| Genre/form data or focus term | Electronic book. |
| 655 #2 - INDEX TERM--GENRE/FORM | |
| Genre/form data or focus term | Laboratory Manual. |
| Authority record control number | (DNLM)D020484 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hoxhaj, Gerta, |
| Relator term | author. |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Print version: |
| Main entry heading | Najafov, Ayaz. |
| Title | PCR guru. |
| Place, publisher, and date of publication | Amsterdam : Academic Press, Elsevier, [2017] |
| International Standard Book Number | 0128042311 |
| -- | 9780128042311 |
| Record control number | (OCoLC)959593099 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Materials specified | ScienceDirect |
| Uniform Resource Identifier | http://www.sciencedirect.com/science/book/9780128042311 |
No items available.