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Protein purification : principles, high resolution methods, and applications / [electronic resource]
by Janson, Jan-Christer.
Material type:
BookSeries: Methods of biochemical analysis: Publisher: Hoboken, N.J. : John Wiley & Sons, ©2011Edition: 3rd ed.Description: 1 online resource (xiv, 517 pages, [8] pages of color plates) : illustrations (some color).ISBN: 9780470940075; 0470940077; 9780470939932; 0470939931.Subject(s): Proteins -- Purification | Chromatographic analysis | Electrophoresis | Proteïnes -- Purificació | Cromatografia | Electroforesi | SCIENCE -- Life Sciences -- Biochemistry | SCIENCE -- Chemistry -- Organic | Chromatographic analysis | Electrophoresis | Proteins -- Purification | Proteine | Chromatographie | Elektrophorese | Reinigung | Trennverfahren | Llibres electrònics | Electronic booksOnline resources: Wiley Online Library
Contents:
Summary: This thoroughly revised and updated third edition provides an overview of the principles and methodology of the most frequently used biochemical separation techniques for proteins used today. Balancing theory, procedures, and applications, it offers professionals and students in biochemistry, organic chemistry, and analytical chemistry quick access to a wide range of important techniques, including: Gel filtration and ion exchange chromatography; Affinity and covalent chromatography, Reversed phase and hydrophobic interaction chromatography, Ligands from combinatorial libraries.
pt. 1. Introduction -- pt. 2. Chromatography -- pt. 3. Other separation methods and related techniques -- pt. 4. Electrophoresis -- pt. 5. Separation method optimization.
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