Includes bibliographical references and index.

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Section A: Basic Principles. Philosophy of Science / James Ladyman -- Ingredients of Experimental Design / Nick Colegrave -- Statistics: A Journey that Needs a Guide / Gordon Drummond -- Section B: Cell and Molecular. Organ Bath Pharmacology / Emma Robinson -- Small Vessel Myography / Tomoko Kamishima, John M Quayle -- Mammalian Cell Cultures: The Example of Airway Epithelial Cell Cultures for Cystic Fibrosis Research / Scott H Randell -- Electron Microscopy (TEM and SEM) / Paul Verkade -- Fluorescence Microscopy / Mark Jepson -- Intracellular 'Sharp' Microelectrode Recording / Helena C Parkington, Harold A Coleman -- Single Electrode Voltage-Clamp (SEVC) / Harold A Coleman, Helena C Parkington -- Patch Clamp Recording / Neil Bannister, Phil Langton -- Production of Antibodies / Elek Molnar -- Immunocytochemistry and Immunohistochemistry / Elek Molnar -- Immunoprecipitation (IP) / David Bates -- Immunoblotting (Western) / Samantha F Moore, Joshua S Savage, Ingeborg Hers -- Applications of Green Fluorescent Protein (GFP) / Mark Jepson -- Fluorescent Measurement of Ion Activity in Cells / Helen Kennedy -- Detection of Exocytosis : Real Time / Anja Teschemacher -- Viral Vector Transgenesis / Anja Teschemacher -- Polymerase Chain Reaction (PCR) and Reverse Transcription (RT)-PCR / Lucy F Donaldson -- Hybridisation (ISH) / Lucy F Donaldson -- Methods of Interference (Antisense, siRNAs and Dominant Negative Mutations) / Allison Fulford -- Transcriptome Analysis: Microarrays / Charles Hindmarch -- Experimental Proteomics / Thierry Le Bihan -- Section C: . Behavioural Methodology / Emma Robinson -- Genetically Modified Mouse Models / Nina Balthasar -- Wireless Recording of Cardiovascular Signals / Julian FR Paton, Fiona D McBryde -- Electrical Stimulation Methods / Jon Wakerley -- Extracellular Recording / Jon Wakerley -- Antidromic Identification / Jon Wakerley -- Event-Triggered Averaging, Including Spike-Triggered Averaging / Richard Apps -- Axonal Transport Tracing of CNS Pathways / John Crabtree -- Cardiovascular Methods: General Considerations for Human Studies / Erica A Wehrwein, Michael J Joyner -- Measuring Cardiac Output in Humans / Erica A Wehrwein, Michael J Joyner -- Measuring Peripheral Blood Flow in Humans / Erica A Wehrwein, Michael J Joyner.