000 04554cam a2200733Ma 4500
001 ocn769189204
003 OCoLC
005 20171115123136.0
006 m o d
007 cr cn|||||||||
008 110927s2012 njua ob 001 0 eng d
010 _z 2011040418
020 _a9781118147146
_q(electronic bk.)
020 _a1118147146
_q(electronic bk.)
020 _a0470610743
020 _a9780470610749
020 _a9781118147177
_q(electronic bk.)
020 _a1118147170
_q(electronic bk.)
020 _a9781118147160
_q(electronic bk.)
020 _a1118147162
_q(electronic bk.)
020 _z9780470610749
028 0 1 _aEB00062766
_bRecorded Books
029 1 _aAU@
_b000053284818
029 1 _aDEBBG
_bBV040092110
029 1 _aDEBBG
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029 1 _aDEBBG
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029 1 _aNZ1
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035 _a(OCoLC)769189204
_z(OCoLC)761321891
_z(OCoLC)762100530
_z(OCoLC)816871889
_z(OCoLC)860516270
_z(OCoLC)864906067
_z(OCoLC)961637134
_z(OCoLC)962667987
037 _a10.1002/9781118147177
_bWiley InterScience
_nhttp://www3.interscience.wiley.com
040 _aE7B
_beng
_epn
_cE7B
_dYDXCP
_dN$T
_dREDDC
_dOCLCQ
_dDEBSZ
_dOCLCQ
_dOSU
_dOCLCQ
_dOCLCF
_dOCLCQ
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049 _aMAIN
050 4 _aQH588.S83
_bP75 2012eb
060 4 _aQU 325
072 7 _aMED
_x106000
_2bisacsh
082 0 4 _a616.02774
_223
245 0 0 _aPrimary and stem cells : gene transfer technologies and applications /
_cedited by Uma Lakshmipathy, Bhaskar Thyagarajan.
_h[electronic resource]
260 _aHoboken, NJ :
_bJohn Wiley and Sons,
_c©2012.
300 _a1 online resource (xiii, 279 pages) :
_billustrations
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
504 _aIncludes bibliographical references and index.
505 0 _aDNA assembly technologies based on homologus recombination / Billyana Tsvetanova [and others] -- Multigene assembly for construction of synthetic operons : creation and delivery of an optimized all-in-one expression construct for generating mouse iPS cells / Takefumi Sone [and others] -- Strategies for the delivery of naked DNA / Jennifer C. Moore and Ronald P. Hart -- Episomal vectors / Junying Yu -- Nonintegrating DNA virus / Chris Kemp and David Kuninger -- Nonintegrating RNA viruses / Noemi Fusaki -- Protein delivery / Xiao-Jian Han and Kazuhito and Tomizawa -- Sleeping beauty transposon-mediated stable gene delivery / Andrew Wilber [and others] -- Integrating viral vectors for gene modifcations / Gemma L. Mendel, Brian Paszkiet, and Rafal P. Witek -- Bacteriophase integrases for site-specific integration / Bhaskar Thyagarajan -- Improving gene targeting efficiency in human pluripotent stem cells / Sangyoon Han [and others] -- Modifed stem cells as disease models and in toxicity screening / Catharina Ellerstrom [and others] -- Screening and drug discovery / Elizabeth A. Davenport and Robert S. Ames.
520 _a"This book describes basic cell engineering methods, emphasizing stem cell applications, and use of the genetically modified stem cells in cell therapy and drug discovery. Together, the chapters introduce and offer insights on new techniques for engineering of stem cells and the delivery of transgenes into stem cells via various viral and non-viral systems. The book offers a guide to the types of manipulations currently available to create genetically engineered stem cells that suit any investigator's purpose, whether it's basic science investigation, creation of disease models and screens, or cells for therapeutic applications"--Provided by publisher.
650 0 _aCells.
650 0 _aStem cells.
650 2 _aGene Transfer Techniques.
650 2 _aStem Cells.
650 2 _aGenetic Engineering.
650 2 _aTransgenes.
650 7 _aMEDICAL
_xResearch.
_2bisacsh
650 7 _aCells.
_2fast
_0(OCoLC)fst00850250
650 7 _aStem cells.
_2fast
_0(OCoLC)fst01132975
655 4 _aElectronic books.
700 1 _aLakshmipathy, Uma.
700 1 _aThyagarajan, Bhaskar.
776 0 8 _iPrint version:
_tPrimary and stem cells.
_dHoboken, NJ : John Wiley and Sons, ©2012
_w(DLC) 2011040418
856 4 0 _uhttp://onlinelibrary.wiley.com/book/10.1002/9781118147177
_zWiley Online Library
942 _2ddc
_cBK
999 _c205468
_d205468