000 06908cam a2200817 i 4500
001 ocn881064963
003 OCoLC
005 20171030094732.0
006 m o d
007 cr |||||||||||
008 140604s2014 nju ob 001 0 eng
010 _a 2014022003
016 7 _a016890425
_2Uk
020 _a9781118336113
_q(ePub)
020 _a1118336119
_q(ePub)
020 _a9781118336137
_q(Adobe PDF)
020 _a1118336135
_q(Adobe PDF)
020 _a9781118336144
020 _a1118336143
020 _a1322008426
020 _a9781322008424
020 _a0470659858
_q(cloth)
020 _a9780470659854
_q(cloth)
020 _z9780470659854
_q(cloth)
028 0 1 _aEB00595503
_bRecorded Books
029 1 _aCHBIS
_b010208947
029 1 _aCHVBK
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029 1 _aDEBSZ
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029 1 _aNLGGC
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029 1 _aNZ1
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029 1 _aGBVCP
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029 1 _aDEBBG
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035 _a(OCoLC)881064963
_z(OCoLC)885019005
_z(OCoLC)904961748
_z(OCoLC)927507921
037 _a632093
_bMIL
040 _aDLC
_beng
_erda
_epn
_cDLC
_dYDX
_dN$T
_dOCLCF
_dDG1
_dYDXCP
_dUKMGB
_dIDEBK
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042 _apcc
049 _aMAIN
050 0 0 _aTD427.F68
070 0 _aTD427.F68
_bB564 2014
072 7 _aTEC
_x010000
_2bisacsh
082 0 0 _a628.9/6
_223
245 0 0 _aBiofouling methods /
_cedited by Sergey Dobretsov, Jeremy C. Thomason, David N. Williams.
_h[electronic resource]
250 _aFirst edition.
264 1 _aHoboken, NJ :
_bWiley-Blackwell,
_c2014.
300 _a1 online resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
500 _aIncludes index.
504 _aIncludes bibliographical references and index.
505 0 _a""Title page""; ""Copyright page""; ""List of contributors""; ""Introduction""; ""Guide to methods""; ""Part I: Methods for Microfouling""; ""1 Microscopy of biofilms""; ""Section 1: Traditional light and epifluorescent microscopy""; ""1.1 Introduction""; ""1.2 Determination of bacterial abundance""; ""1.3 Catalyzed reporter deposition fluorescent in situ hybridization (CARD-FISH)""; ""1.4 Suggestions, with examples, for data analysis and presentation""; ""Acknowledgements""; ""References""; ""Section 2: Confocal laser scanning microscopy""; ""1.5 Introduction""
505 8 _a""1.6 Materials, equipment, and method""""1.7 Image acquisition""; ""1.8 Presentation""; ""1.9 Troubleshooting hints and tips""; ""1.10 Notes""; ""References""; ""Section 3: Electron microscopy""; ""1.11 Introduction""; ""1.12 Transmission electron microscopy (TEM)""; ""1.13 Scanning electron microscopy (SEM)""; ""References""; ""2 Traditional and bulk methods for biofilms""; ""Section 1: Traditional microbiological methods""; ""2.1 Introduction""; ""2.2 Enrichment culture, isolation of microbes""; ""2.3 Counting methods""; ""2.4 Troubleshooting hints and tips""; ""References""
505 8 _a""Section 2: Bulk methods""""2.5 Introduction""; ""2.6 Measurement of biofilm thickness""; ""2.7 Biofilm dry weight determination""; ""2.8 Biofilm ATP content""; ""2.9 Troubleshooting hints and tips""; ""Acknowledgements""; ""References""; ""3 Biocide testing against microbes""; ""Section 1: Testing biocides in solution""; ""3.1 Introduction""; ""3.2 Method introductions""; ""3.3 Pros and cons""; ""3.4 Materials and equipment""; ""3.5 Methods""; ""3.6 Troubleshooting hints and tips""; ""3.7 Suggestions""; ""References""; ""Section 2: Biocide testing using single and multispecies biofilms""
505 8 _a""3.8 Introduction""""3.9 Questions to answer when applying biocides""; ""3.10 Laboratory methods for testing biocide effect""; ""3.11 Field methods for testing biocide effect""; ""3.12 Troubleshooting hints and tips""; ""Acknowledgements""; ""References""; ""4 Molecular methods for biofilms""; ""Section 1: Isolation of nucleic acids""; ""4.1 Introduction""; ""4.2 Materials""; ""4.3 Isolation of DNA from a biofilm""; ""4.4 Troubleshooting hints and tips""; ""References""; ""Section 2: PCR and DNA sequencing""; ""4.5 PCR and DNA sequencing: General introduction""; ""4.6 PCR""
505 8 _a""4.7 Microbial marker genes â€" 16S""""4.8 DNA sequencing""; ""4.9 454 16S amplicon pyrotag sequencing""; ""4.10 Protocol 1: DNA extraction using the Qiagen DNeasy Plant Mini Kit""; ""4.11 Protocol 2: Full-length 16S PCR using the Qiagen Multiplex Kit""; ""4.12 Protocol 3: Analysis of full-length 16S genes""; ""4.13 Protocol 4: 16S amplicon PCR for 454 sequencing using the Qiagen Multiplex Kit""; ""4.14 Protocol 5: Trimming and filtering of 454 16S pyrotag sequencing""; ""4.15 Protocol 6: Taxon-based analyses""; ""4.16 Protocol 7: Phylogeny-based analyses""; ""References""
520 _aBiofouling Methods provides a "cook book" forboth established workers and those new to the field. The methodsincluded in this important new book range from tried and testedtechniques to those at the cutting edge, encompassing the fulldiversity of this multidisciplinary field. The book covers methods for microbial and macrofouling, coatingsand biocides, and ranges from methods for fundamental studies tomethods relevant for industrial applications. There is an emphasison answering questions and each chapter provides technical methodsand problem-solving hints and tips. Bringing together a wealth of international contributions andedited by three internationally known and respected experts in thesubject Biofouling Methods is the essential methodologyreference in the field for all those working in the antifoulingindustry including those involved in formulation of antifoulingproducts such as paints and other coatings. Aquatic biologists, ecologists, environmental scientists and lawyers, marine engineers, aquaculture personnel, chemists, and medical researchers will allfind much of interest within this book. All universities andresearch establishments where these subjects are studied and taughtshould have copies of this important work on their shelves.
588 0 _aPrint version record and CIP data provided by publisher.
650 0 _aFouling.
650 0 _aFouling organisms.
650 4 _aFouling organisms.
650 7 _aTECHNOLOGY & ENGINEERING
_xEnvironmental
_xGeneral.
_2bisacsh
650 7 _aFouling.
_2fast
_0(OCoLC)fst00933249
650 7 _aFouling organisms.
_2fast
_0(OCoLC)fst00933253
655 4 _aElectronic books.
700 1 _aDobretsov, Sergey.
700 1 _aThomason, Jeremy.
700 1 _aWilliams, David N.
_q(David Neil),
_d1966-
776 0 8 _iPrint version:
_tBiofouling methods.
_bFirst edition.
_dHoboken, NJ : John Wiley & Sons, Inc., 2014
_z9780470659854
_w(DLC) 2014018424
856 4 0 _uhttp://onlinelibrary.wiley.com/book/10.1002/9781118336144
_zWiley Online Library
942 _2ddc
_cBK
999 _c207492
_d207492