| 000 | 05817cam a2200553Ii 4500 | ||
|---|---|---|---|
| 001 | ocn945095030 | ||
| 003 | OCoLC | ||
| 005 | 20190328114814.0 | ||
| 006 | m o d | ||
| 007 | cr cnu|||unuuu | ||
| 008 | 160318t20162016ne a ob 001 0 eng d | ||
| 040 |
_aN$T _beng _erda _epn _cN$T _dN$T _dYDXCP _dN$T _dOPELS _dOCLCF _dCDX _dCOO _dEBLCP _dIDEBK _dIDB _dDEBSZ _dCNCGM _dMERUC _dOCLCQ _dOCLCO _dU3W _dUUM _dWRM _dD6H _dORE _dOCLCQ |
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| 019 |
_a945567083 _a945613192 |
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| 020 |
_a9780128016848 _q(electronic bk.) |
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| 020 |
_a0128016841 _q(electronic bk.) |
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| 020 | _a012801394X | ||
| 020 | _a9780128013946 | ||
| 020 | _z9780128013946 | ||
| 035 |
_a(OCoLC)945095030 _z(OCoLC)945567083 _z(OCoLC)945613192 |
||
| 050 | 4 | _aQH581.2 | |
| 072 | 7 |
_aSCI _x056000 _2bisacsh |
|
| 082 | 0 | 4 |
_a571.6 _223 |
| 100 | 1 |
_aCasem, Merri Lynn, _eauthor. |
|
| 245 | 1 | 0 |
_aCase studies in cell biology / _h[electronic resource] _cMerri Lynn Casem. |
| 264 | 1 |
_aAmsterdam : _bAcademic Press is an imprint of Elsevier, _c[2016] |
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| 264 | 4 | _c�2016 | |
| 300 |
_a1 online resource (xix, 384) : _billustrations (some color). |
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| 336 |
_atext _btxt _2rdacontent |
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| 337 |
_acomputer _bc _2rdamedia |
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| 338 |
_aonline resource _bcr _2rdacarrier |
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| 490 | 1 | _aProblem sets in biological and biomedical sciences | |
| 504 | _aIncludes bibliographical references and index. | ||
| 588 | 0 | _aOnline resource; title from PDF title page (EBSCO, viewed March 23, 2016). | |
| 520 | _a"Case Studies in Cell Biology presents real world scenarios to help readers use science process and reasoning skills. The case studies require application and analyzation of concepts beyond rote memory of biological concepts. The book is based on the student learning outcomes from the American Society for Cell Biology, offering practical application for both the classroom and research laboratory. Guides the reader in applying knowledge directly to real world scenarios Includes case studies to bridge foundational cell biological concepts to translational science Aids students in synthesizing information and applying science processes." | ||
| 505 | 0 | _aCover; Title Page; Copyright Page; Dedication; Contents; Biography; Preface; Chapter 1 -- Introduction to Scientific Method from a Cellular Perspective; Subchapter 1.1 -- Scientific Method in Action [1]; Introduction; Background; Methods; Cell cultures; Microinjection; Electron microscopy; Calculation of nuclear uptake and size distribution; Results; Reference; Chapter 2 -- Cellular Biodiversity; Subchapter 2.1 -- Cellular Biodiversity on the High Seas [1]; Introduction; Background; Methods; Cell culture and population density; Characterization of photosynthetic pigments; Results. | |
| 505 | 8 | _aSubchapter 2.2 -- The Mystery of the Missing Mitochondria [2]Introduction; Background; Methods; Subcellular fractionation of giardia; Transmission electron immunomicroscopy; Results; References; Chapter 3 -- Proteins; Subchapter 3.1 -- Bite of the Brown Recluse Spider: An Introduction to Protein Gel Electrophoresis [1]; Introduction; Background; Methods; Red blood cell ghosts; Venom digestion assays; Protein gel electrophoresis; Results; Subchapter 3.2 -- Where to Start? A Case of Too Many AUGs [3]; Introduction; Background; Methods; Yeast culture conditions. | |
| 505 | 8 | _aCDC9-GFP fusions, CDC9 mutagenesis, and HA taggingSubcellular fractionation and protease K digestion; Immunoblotting; Results; Subchapter 3.3 -- Cotranslational Translocation: Gatekeepers of the RER [4]; Introduction; Background; Methods; In vitro translation; Crosslinking assay; Flotation assay; Protease resistance assay; Results; Subchapter 3.4 -- From Sequence to Function [5]; Introduction; Background; Methods; Isolation and structural analysis of bovine AQP1; Data processing; Results; Subchapter 3.5 -- Visualizing Protein Conformation [6]; Introduction; Background; Methods. | |
| 505 | 8 | _aProtein expression and purificationElectron microscopy; SNARE complex labeling; Results; Subchapter 3.6 -- Second Chance Chaperones: How Misfolded Proteins get Refolded [7]; Introduction; Background; Methods; Purification of Hsp104; Purification of Hsp40 and Hsp70; Preparation of yeast lysates; Turbidity assay; Refolding assays; Gel filtration; Results; References; Chapter 4 -- The Nucleus; Subchapter 4.1 -- Nuclear Pore Complex Assembly is a House of Cards [1]; Introduction; Background; Methods; Nuclear reconstitution assay; Immunodepletion; Nuclear import; Immunoblotting; DNA replication assay. | |
| 505 | 8 | _aScanning electron microscopyResults; Subchapter 4.2 -- Importin b: The Important Importin [2]; Introduction; Background; Methods; Preparation of a high-speed supernatant from Xenopus eggs; Immunodepletion of importin from cytosol; Nuclear protein import assay; Nuclear localization signal binding assay; Protein gel electrophoresis and immunoblotting; Results; Subchapter 4.3 -- A Tale of tRNA Transport [3]; Introduction; Background; Methods; In vitro translation and 35S-labeling; Injection into Xenopus frog eggs; In vitro binding assays; Gel electrophoresis and immunoblotting; Results. | |
| 505 | 8 | _aSubchapter 4.4 -- When It Comes to the Nucleus -- Size Matters [4]. | |
| 650 | 0 |
_aCytology _vCase studies. |
|
| 650 | 7 |
_aSCIENCE _xLife Sciences _xAnatomy & Physiology. _2bisacsh |
|
| 650 | 7 |
_aCytology. _2fast _0(OCoLC)fst00886282 |
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| 655 | 4 | _aElectronic books. | |
| 655 | 7 |
_aCase studies. _2fast _0(OCoLC)fst01423765 |
|
| 776 | 0 | 8 |
_iPrint version: _aCasem, Merri Lynn. _tCase studies in cell biology. _dLondon, England : Academic Press, �2016 _hxix, 384 pages _kProblem Sets in Biological and Biomedical Sciences series _z9780128013946 |
| 830 | 0 | _aProblem sets in biological and biomedical sciences. | |
| 856 | 4 | 0 |
_3ScienceDirect _uhttp://www.sciencedirect.com/science/book/9780128013946 |
| 999 |
_c247304 _d247304 |
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