000			05538cam a2200601Ii 4500
001			ocn965140915
003			OCoLC
005			20190328114817.0
006			m o d
007			cr |n|||||||||
008			161202t20172017ne ob 001 0 eng d
040			_aYDX _beng _erda _epn _cYDX _dOCLCQ _dNLE _dOCLCO _dIDEBK _dOPELS _dN$T _dEBLCP _dGZM _dSTF _dIDB _dDEBSZ _dOCLCF _dTFW _dN$T _dGZM _dOCLCQ _dVGM _dOCLCQ _dOCL _dMERUC _dCNCGM _dU3W _dUAB _dCOO _dOTZ _dOCLCO _dOCL _dMERER _dORE _dOCLCO _dD6H _dOCLCQ _dCHVBK _dBRX _dOCLCQ _dOCLCO _dWYU _dOCLCO _dOCLCA
016	7		_a101702242 _2DNLM
019			_a964656833 _a967076811 _a971588839 _a971589167 _a971918018 _a1066596587
020			_a9780128042465 _q(electronic bk.)
020			_a012804246X _q(electronic bk.)
020			_z0128042311
020			_z9780128042311
035			_a(OCoLC)965140915 _z(OCoLC)964656833 _z(OCoLC)967076811 _z(OCoLC)971588839 _z(OCoLC)971589167 _z(OCoLC)971918018 _z(OCoLC)1066596587
050		4	_aQP606.D46
060	0	0	_a2017 B-894
060	1	4	_aQU 25 _bN162p 2017
072		7	_aSCI _x007000 _2bisacsh
082	0	4	_a572.33 _223
100	1		_aNajafov, Ayaz, _eauthor.
245	1	0	_aPCR guru : an ultimate benchtop reference for molecular biologists / _h[electronic resource] _cAyaz Najafov, Gerta Hoxhaj.
264		1	_aAmsterdam : _bAcademic Press, Elsevier, _c2017
264		4	_c�2017
300			_a1 online resource (xv, 89 pages)
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
500			_aPublisher, Sara Tenney ; acquisitions editor, Sara Tenney ; editorial project manager, Fenton Coulthurst ; production project manager, Chris Wortley ; designer, Christian Bilbow.
504			_aIncludes bibliographical references pages 83-86) and index.
505	0		_aCover; Title Page; Copyright Page; Dedication; Contents; Biography; Preface; Acknowledgments; Note to the Reader; Chapter 1 -- Introduction; Abstract; 1.1 -- A bit of history; 1.2 -- What is PCR?; 1.3 -- PCR components; 1.4 -- Exponential nature of PCR; 1.5 -- Popularity of PCR; Chapter 2 -- Procedure; Abstract; 2.1 -- Primer design; 2.2 -- Standard PCR setup; 2.3 -- Controls; 2.4 -- Cycling parameters; 2.4.1 Denaturation; 2.4.2 Annealing; 2.4.3 Extension; Chapter 3 -- Good PCR Practices; Abstract; Chapter 4 -- Optimization and Troubleshooting; Abstract; 4.1 -- Optimization rules.
505	8		_a4.2 -- General optimization strategies4.2.1 -- Annealing Temperature Gradient; 4.2.2 -- Mg++ Titration; 4.2.3 -- Hot Start; 4.2.4 -- Touchdown PCR; 4.2.5 -- Additives; 4.3 -- Troubleshooting specific problems; 4.3.1 -- Problem Type 1; 4.3.2 -- Problem Type 2; 4.3.3 -- Problem Type 3; 4.3.4 -- Problem Type 4; 4.3.5 -- Problem Type 5; 4.3.6 -- Other Problem Types; Chapter 5 -- Tips and Tricks; Abstract; Chapter 6 -- Special Cases; Abstract; Glossary and Abbreviations; A; B; C; D; E; G; K; M; N; O; P; Q; R; S; T; X; Appendix A -- Agarose Gel Electrophoresis Protocol; Appendix B -- Silver Staining of DNA Gels.
505	8		_aAppendix C -- Plasmid Miniprep Protocol (by using "QIAprep Spin Miniprep" Kit)Appendix D -- Restriction Enzyme Digestion Protocol; Appendix E -- Protocol for DNA Concentration Estimation Using Hoechst 33258; Appendix F -- Formulas; Appendix G -- Common Primers for Sequencing, Promoters, Housekeeping Genes, and Reporters; Appendix H -- Tags; Appendix I -- Thermostable DNA polymerases; Appendix J -- Example of a Daily PCR Log; Appendix K -- Common Buffers and Solutions; Appendix L -- Useful Numbers; Appendix M -- Yeast Colony PCR; Useful Websites; References; Index; Back Cover.
520			_a"PCR Guru: An Ultimate Benchtop Reference for Molecular Biologists is provides researchers in molecular biology with a handy reference for approaching and solving challenging problems associated with PCR setup and optimization. As a laboratory guide, it emphasizes the technical aspects of employing PCR as a tool in molecular biology laboratories. The book covers the history of PCR and the basic science underlying it. It then discusses PCR at the bench level, starting with detailed description and tips on primer design, and continuing with the standard protocols used to perform PCR. Provides troubleshooting tips for various types of modifications of standard protocols Contains unique "Good Practices and Tips that are indispensable for the beginner and expert alike Features "Special Cases with applications of PCR, optimization, and troubleshooting Includes detailed appendices with tables, figures, and key protocols Organized as a systematic, concentrated resource to save time when addressing a PCR problem."
588	0		_aOnline resource; title from PDF title page (ScienceDirect, viewed February 16, 2017).
650		0	_aPolymerase chain reaction.
650		0	_aMolecular biology.
650		7	_aSCIENCE _xLife Sciences _xBiochemistry. _2bisacsh
650		7	_aMolecular biology. _2fast _0(OCoLC)fst01024734
650		7	_aPolymerase chain reaction. _2fast _0(OCoLC)fst01070553
650	1	2	_aPolymerase Chain Reaction _xmethods. _0(DNLM)D016133Q000379
650	2	2	_aMolecular Biology. _0(DNLM)D008967
655		4	_aElectronic books.
655		0	_aElectronic book.
655		2	_aLaboratory Manual. _0(DNLM)D020484
700	1		_aHoxhaj, Gerta, _eauthor.
776	0	8	_iPrint version: _aNajafov, Ayaz. _tPCR guru. _dAmsterdam : Academic Press, Elsevier, [2017] _z0128042311 _z9780128042311 _w(OCoLC)959593099
856	4	0	_3ScienceDirect _uhttp://www.sciencedirect.com/science/book/9780128042311
999			_c247486 _d247486